1980-1985 Very low platelet counts are common in cancer and until this invention had been impossible to measure
Hansen developed laser platelet counter. First clinical method for platelet enumeration in cancer patients
US Patent 4,577,964. The use of laser optics for high sensitivity counting of small cells at low count levels
1985-1987 Cells can be immobilized in micro-arrays, however this invention allowed cells to be selected and moved to specific positions in a micro-array
Hansen developed first micro-array for selecting and transfecting cells
US Patent 4,661,451. The use of an electronic micro-array to translocate, immobilize and transfect cells
1989-1995 This invention used a flow cytometer of extremely small dimensions to give physicians rapid results from a TORCH panel
Hansen developed first point of care flow cytometer (FDA cleared). Device to give physicians TORCH panel results in 5 minutes
US Patents 5,286,452; 5,369,037; 6,200,820
1995-1998 Veterinarians needed a flow cytometer/hematology analyzer for field use and use with multiple species
Hansen developed first multi-species flow cytometer for veterinary diagnostics; Five-part differential hematology system for field use.
US Patents 6,320,656; 6,618,143; 7,064,823
1997-2002 At the same time that the c.elegans genome was sequenced, Hansen developed a flow cytometer/sorter that would select these organisms, in vivo, based on gfp expression
Hansen developed first flow cytometer to analyze and sort c. elegans in vivo.
Used fluorescent proteins to mark gene expression and sort organisms at >500 per second
US Patents 6,400,453; 6,657,713; 7,116,407
1979-1981: The first applications for CD4 counting were in transplant patients and later for AIDS. By combining light scatter and fluorescence, Hansen co-invented the first automated CD4 system, which became the basis for all flow cytometry CD4 counting in use today.
Hansen developed the first automated flow cytometry system for clinical immunophenotyping used clinically to monitor immune-suppression in transplantation patients. First automated method for CD4 counting in AIDS.
US Patent 4,284,412. The combined use of light scatter and fluorescence gates for immunophenotyping cells in flow cytometry
Use of monoclonal antibodies to T-cell subsets for immunologic monitoring and treatment in recipients of renal allografts; Cosimi, Colvin, Burton, Rubin, Goldstein, Kung, Hansen, Delmonico, and Russell; The New England Journal of Medicine (1981) vol 305, Issue 6, pp308-314
Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood; Hoffman, Hansen, and Goldstein; Proceedings of the National Academy of Sciences, 1980, August 77(8) pp 4914-491
1980-1982: Using flow cytometry, Hansen automated the reticulocyte count, which until that time had been inaccurate and not useful for anemia diagnostics
Hansen developed the first instrument-based method for reticulocyte counting First accurate detection of therapeutic efficacy in anemia
US Patent 4,325,706. The first instrument-based hematology method for reticulocyte counting
1980-1981: Red cell volume is a fundamental parameter in hematology but until this development by Hansen, red cell shape abnormalities introduced errors in this measurement
Hansen developed first shape-independent red cell volume method based on basic studies of red cell volume measurement.
1981- US Patent 4,284,355. The use of fluorescence-exclusion red cell volume measurement